Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Small Intestinal Permeability and Gut-Transit Time Determined MeSH terms The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. 2005;22(4):5505. Int J Pharm Investig. Schematic illustration of transdermal delivery after the application of the microporation device. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. dextran Environ Toxicol Pharmacol. These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Elias, Hans-Georg (1975). Dextrans in Aqueous Solution. Experimental Review on Intrinsic Colloid and Polymer Science, 286, 1223-1231. Physicochemical studies of oligodextran. Bohdanecky, Miloslav (1996). By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. Charge: Anionic. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Webdextran hydrodynamic radius. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. Federal government websites often end in .gov or .mil. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. The skin poration was performed using the microporation device (LG electronics, Korea). The Hydrodynamic Radii of Macromolecules and Their 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Thermo Fisher Scientific. Front Microbiol. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. The Transdermal diffusion cell system was protected from light. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Ficoll and dextran vs. globular proteins as probes for testing It is well tolerated on parenteral infusion by most people but a very low incidence The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. ; Picout, D.R. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Critical parameters for gel permeation He, L.; Niemeyer, B. A solvent at this temperature is called a theta solvent [53]. 2015;23(3):2239. Dextran. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. dextran ; Dutt, N.V.K. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. The effect of branching is best recognized from the molecular weight dependencies of []. 2011;5(2):95132. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. De Belder, A.N. (2001). For Research Use Only. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). dextran hydrodynamic radius Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in The mechanism of the dextran-induced red blood cell Aggregation. (2007). In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Gekko, K. (1971). AATEX. Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. The human skins were from the back or thigh of a 24-year-old woman. Biophysical Journal, 27, 177-181. Carbohydrate Polymers 95 360-365. Progress in Biophysical Molecules Biological, 68, 207-262. Ioan, C.E. Biomaterials. Hennink, W.E. J Appl Toxicol. Journal of Chemical Education, 80(9), 1036-1038. Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) (2003). Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. In fact, short chains do contain minor branching points. Hydrodynamic Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. 2016;9706:97061E6. Drug Deliv Syst. CAS Journal of Colloid Science, 31, 308-328. Proc. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Influence of molecular weight on statement and Search For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Differences were considered statistically significant at p <0.05. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). Correspondence to Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Colloid Polym. Regulating dynamic signaling between hematopoietic stem cells Guner, A.; Kibarer, G. (2001). Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Accessibility TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. Dextran Fractions. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). Dextran (2003). Carbohydrate Polymers, 39, 377-380. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Morris, G.A. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Biomaterials Research WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Drug Discov Today Technol. Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. 151-166. Rotureau, E.; Dellacherie, E.; Durand, A. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. ; Bataille, B. Human skins (Full-thickness human skin) were supplied by HansBiomed. (2008). 2006;3(3):37385. Journal of Polymer Science, 17, 527-546. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Keywords: FITC dextran; intestinal permeability; transit time. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. The receptor chamber was filled with PBS. Polysaccharide hydrogels for modified release formulations. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. Degrees of long-chain branching in dextrans. Springer Editions. sharing sensitive information, make sure youre on a federal However, in vitro investigations are very few. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Dextrans in Aqueous Solution. Experimental Review on Intrinsic Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Synthesis and characterization of blue dextrans WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Biomacromolecules 8, 2464-2475. Journal of Controlled Release, 119, 5-24. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Clipboard, Search History, and several other advanced features are temporarily unavailable. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Macromolecules, 29, 2265-2268. American Journal of Food Science and Technology 2013, 1(3), 60-66. J Pharm Sci. of different origins. dextran hydrodynamic 113, 81-86. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Recent Pat Drug Deliv Formul. Journal Non-Newtonian Fluid Mech., 97, 295-301. Carbohydrate Polymers, 74, 372-378. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. Harding, Stephen E. (2005). Hydrodynamic The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Hydrodynamic Radius Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. WebTry out PMC Labs and tell us what you think. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. Analytical Chemistry. Carbohydrate Polymers, 36, 319-326. 2008;28(5):64557. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the We observe that this maximum radius is below Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Advanced Drug Delivery Reviews, 60, 1650-1662. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. ; Hogan, M.P. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Bethesda, MD 20894, Web Policies Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Transdermal drug delivery: overcoming the skins barrier function. Sachdeva V, Banga AK. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. 2012;101(8):28619. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. FOIA European Polymer Journal, 36, 2143-2146. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. Intrinsic viscosity-molecular weight relationship for chitosan. Epub 2021 Feb 9. Keywords: Each microporated sample was incubated in the skin culture medium. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. ; De Jong, S.J. If there are particles in the sample, the incident laser light gets scattered in all directions. Huggins equation [41] gives the relation to the relative viscosity increase (i). Soc., 16, 3A, 426-433. Disclaimer. WebFITC Products. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. ; Bos, G.W. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). This study was supported by the LG Electronics. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). ; Grishchenko, A.E. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. The Mark-Houwink. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with